InferLOH: Assessing CRISPR-Induced Copy-Neutral Loss-of-Heterozygosity Prevalence Through Single-Cell RNA Sequencing
Time: 9:00 am
day: Conference Day Two
Details:
- CRISPR-based genome editing may lead to copy-neutral loss-of-heterozygosity (LOH) occurring from the site of CRISPR editing to the telomere of the same chromosome arm. LOH could have serious health-related implications
- Most existing LOH assessment methods rely on cell cloning, which may not be applicable for primary cells. In addition, the methods are usually labor- and time intensive
- We have developed a single-cell RNA sequencing (scRNAseq) and machine learning based method that accurately predicts the zygosity of a genomic DNA region of interest of individual cells
- This high-throughput method also allows us to assay large number of cells to reduce sampling error in the estimation of the LOH prevalence in a cell population
