Developing a Framework for Off-Target Prediction & Detection

Time: 11:00 am - 1:00 pm
day: Pre-Conference Workshop Day


  • Summarizing the most popular methods for off-target nomination and detection: their relative strengths and weaknesses
  • What should I consider when choosing, and how do they complement bioinformatic nomination?
  • Using appropriate read depth and window size to generate high assay sensitivities
  • Within the current regulatory context, how can these tools be applied for the risk assessment of a gene editing tool?
  • Understanding modifications to off-target testing requirements in the recently finalized FDA guidance: how to implement this framework in a real-world situation